2012;29(7):201729. (2001). However, in vitro investigations are very few. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. ; Weisz, J. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. Chen Rong Huei; Wei Yu Chen; Shang Ta Wang; Chu Hsi Hsu; Min Lang Tsai (2009). Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. (2006). Anal. Manage cookies/Do not sell my data we use in the preference centre. Keywords: FITC dextran; intestinal permeability; transit time. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. European Polymer Journal, 37, 619-622. Figure4 shows the histological analysis of micropores in human cadaver skin. This study demonstrated that hydrodynamic volume and radius of gyration of a dextran sample with M w < 20 kDa and its linear counterpart with equal M w are almost WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. 2004;25(9):155967. These hydrodynamic parameters show that these polysaccharides behave as a compact rigid sphere and contract by the increase of temperature (RH decreases) for the Mw range from 8.8 to 200kDa. The authors declare that they have no competing interests. Kumar SV, Tarun P, Kumar TA. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. It is well tolerated on parenteral infusion by most people but a very low incidence Chen Rong Huei; Min Lang Tsai (1998). Journal of American Chemical Society, 76 (20), 5041-5052. Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. Harding, Stephen E. (2005). 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. ; Fran, H.P. The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. Would you like email updates of new search results? The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. Herwadkar A, Banga AK. The mechanism of the dextran-induced red blood cell Aggregation. Accessibility Carbohydrate Research, 160, 205-214. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. Ther. The hydrodynamic radius of 2-NBDG glucose was too small to measure. After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. eCollection 2015. NCI CPTC Antibody Characterization Program. Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta In vivo iontophoretic delivery of salmon calcitonin across microporated skin. The slope is a value and intercept is equal to the ln kMH value [47]. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. 2009;8(2):1739. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. 2000;3(9):31826. Masuelli, M. A. American Journal of Food Science and Technology 2013, 1(3), 60-66. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. Chem. Durand, A. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. 2022 Feb 22;16(2):1999-2012. doi: 10.1021/acsnano.1c07010. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Saudi Pharm J. GPC Symp. 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. Carbohydrate Polymers, 79, 380-390. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. ; Talsma, H.; Hennink, W.E. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. The relation between Mw and the intrinsic viscosity is given by Mark-Houwink equation. Journal of Controlled Release, 119, 5-24. Doke SK, Dhawale SC. Smith, David R.; Raymonda, John W. (1972). Google Scholar. Synthesis and characterization of blue dextrans. Environ Toxicol Pharmacol. WebTry out PMC Labs and tell us what you think. 2018;1:10915. Biomater Res 24, 22 (2020). Ioan, C.E. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. This result could reflect the presence of some aggregated species within the lysozyme sample solution. The microporation device based on radiofrequency can be used to enhance delivery efficiency by removing the epidermis layer. A similar combination involves the intrinsic viscosity and specific volume: (a/b) is called Einstein viscosity increment, and Vs is specific volume (cm3/g). Dextrans in Aqueous Solution. Drug Deliv. Pharmacol Pharm. Stenekes, R.J.H. ; Patel, T.R. Drug deliv. (2008). 8. The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. CAS The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. (2013). Cookies policy. In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH, MarkHouwink constants. In general, Mv is not experimentally accessible, whereas other average molecular weights are accessible. Gold MH, Biron JA. European. 2012;101(8):28619. Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. 24h after microporation, the pore sizes reduced from 64.8817.20m (0h) to 48.854.27m (24h), and the pore depths reduced from 83.9120.56m (0h) to 63.9527.61m (24h). Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. Physicochemical studies of oligodextran. (2008). Intrinsic viscosity of bovine serum albumin conformers. ; Garca de , J. Carbohydrate Research, 340, 811-826. However, the results did not show significant regeneration of micropore compared to previous in vivo studies which reported significant regeneration [25]. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. Biomaterials Research Structure Properties of Dextran. ; Picout, D.R. Studies carried out at different ionic strength you can see them in the references [64, 65] and works with different solvents in the references [66, 67]. Determination of polymer structure by Gel Permeation Chromatography. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. De Belder, A. N. (2001). Carbohydrate Polymers 95 360-365. 151-166. government site. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. (2003). Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. (2012). In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). (2004). (1998). 2003;10:33847. A single frequency laser is directed to the sample contained in a cuvette. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Human skins (Full-thickness human skin) were supplied by HansBiomed. Journal of Applied Polymer Science, 27, 795-810. However, considering the trauma and unethical practices associated with animal experiments, in vitro studies with human cadaver skin offers an ethical and viable alternative. Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. Dextran produced from Weissella cibaria JAG8 was purified and characterized. Advances in Carbohydrate Analysis, 1, 63-144. Treatment of acne scars by fractional bipolar radiofrequency energy. Pharm Res. Peptide and protein transdermal drug delivery. Springer Nature. TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. Advanced Drug Delivery Reviews, 60, 1650-1662. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Chem. 2019;9(3-S):9726. Polysaccharides-based nanoparticles as drug delivery systems. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. Biophysical Journal, 87, 4259-4270. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. 2009;301(8):60913. Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). Radiofrequency with microneedling. Banga AK. Terms and Conditions, Dependence on Concentration. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. In a good solvent, a temperature increase results in an intrinsic viscosity decrease and in a less-extended conformation (RH<), because the entropy value increases with an increase in temperature and it is unfavorable for an extended conformation. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Epub 2012 May 28. Figure5 shows the cumulative amount of the FITC-dextran (M.W. Kim, D.; Robyt, J. F.; So-Young Lee; Jin-Ha Lee; Young-Min Kim. Viscotek GmbH, Europe. pp13-21. In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). As a consequence of the diversity in their origin, the set of classical universal size independent quantities suffers from shortcomings. what will you do to keep amazon safe answers; amarsi assisted living; peter clarke, emilia clarke; personality characteristics of kiran mazumdar shaw; karina mitchell age; mirela romanian orphan died; dsa presidential endorsement; dextran hydrodynamic radius. (1954). Bookshelf Guner, A.; Kibarer, G. (2001). Webdextran hydrodynamic radius. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. Soc., 16, 3A, 426-433. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. Characterization and Classification of Dextrans from Ninety-six Strains of Bacteria. Solution properties of branched dextran. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Sci. The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. 1905/05) for their financial support. CAS The micropore formed at the SC through to the epidermis, enabling drug delivery. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). Calculation of MarkHouwinkSakurada (MHS) equation viscometric constants for chitosan in any solventtemperatura system using experimental reported viscometric constants data. Epub 2021 Feb 9. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly Ahn, G.Y., Eo, HS., Kim, D. et al. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. Walkenhorst, S.; Olivier, R. (2002). Formation of dextran hydrogels by crystallization. Porcine wound models for skin substitution and burn treatment. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. Journal of Applied Polymer Science, 13, 2031-2032. Kasaai, Mohammad R. (2012). Huggins equation [41] gives the relation to the relative viscosity increase (i). Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. Determination of intrinsic viscosities of macromolecules and nanoparticles. eCollection 2018 Feb. Molecules. Qi P, Caoa M, Songa L, Chena C, Liua M, Li N, Wua D, Penga J, Hub G, Zhao J. Recent Pat Drug Deliv Formul. HSE and DK helped to porate the skin and to measure TEWL. AATEX. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. This study was supported by the LG Electronics. These studies were carried out at the Hisko (Gunpo, Korea). Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. The solutions were prepared in bidestilated water at 1, 0.75, 0.5, 0.25 % wt. Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). Covis, Rudy; Ladaviere, Catherine; Desbrieres, Jacques; Marie, Emmanuelle; Durand, Alain. Soc., 21, 1, 78-86. 1984;17(6):11703. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Beer, M.U. Table 2 compares the properties of nanoparticles coated with one or other polymer. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. Ghimici, Luminita; Nichifor, Marieta; Wolf, Bernhard (2009). Sachdeva V, Banga AK. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation. 2009;6(4):34354. ; Bos, G.W. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68].
dextran hydrodynamic radius
- Post author:
- Post published:March 17, 2023
- Post category:are camellias poisonous to cattle